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Valley Biomedical Products heat inactivated human ab serum
Heat Inactivated Human Ab Serum, supplied by Valley Biomedical Products, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heat+inactivated+human+ab+serum/ab+human+serum/us12655190-857-32-37
Average 86 stars, based on 1 article reviews
heat inactivated human ab serum - by Bioz Stars, 2026-10
86/100 stars

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Cell Culture:

Article Title: Intercellular nanotube-mediated mitochondrial transfer enhances T cell metabolic fitness and antitumor efficacy.
Article Snippet: NALM6 cell line was originally obtained from DSMZ (ACC 128) and transduced with Luciferase-GFP (GL) as previously described.72 PG13 expressing CD19-CAR (FMC63-28-z) retrovirus was used for transduction of human CD8+ T cells with CD19 CAR as previously described.73 B16KVP melanoma expressing human gp100 was engineered as previously described.38 NALM6-GL and both human and mouse CD8+ T cells were cultured with RPMI complete medium; human BMSCs, PG13 CD19-CAR, B16KVP, Platinum-E, SK23-GFP, and 293GP cells were cultured with DMEM complete medium; and DM5 cell line was cultured with MEM-a complete medium. .. MART-1 TILs were kindly provided by Prof. Lotem and cultured in RPMI supplemented with 10% heat inactivated human AB serum (Valley Biomedical Inc.), 1% Pen/strep (Gibco), 1% HEPES (Gibco) and 0.01% b-mercaptoethanol (Gibco). .. All cell lines were regularly tested and validated as being mycoplasma free via a PCR-based assay (PromoCell).

Article Title: Tn-MUC4 binding polypeptides and uses thereof
Article Snippet: Cellular Immune Response: Peripheral blood mononuclear cells (PBMCs) were isolated from heparin anti-coagulated blood of patients with late stage (III-IV) colorectal cancer using Lymphoprep (Nycomed Pharma AS, Oslo, Norway). .. Cells were then cultured in X-VIVO 15 medium (Invitrogen) together with 2% heat inactivated human AB serum (Valley Biomedical, Winchester, USA) in round bottom microculture plates (Nunc, Roskilde, Denmark). .. 50 IU/ml IL-2 (Proleukin, Chiron, The Netherlands) was added at day 1 and cells were harvested at day 9 and restimulated with the same peptide pools or without peptide as a negative control in an IFN-g ELISPOT assay (Svane, Pedersen et al. 2004) in order to identify MUC-1 specific CD4+ or CD8+ T cells.

Article Title: High efficiency closed-system gene transfer using automated spinoculation
Article Snippet: The CD22 CAR (Lentigen Technology, Gaithersburg, MD) was used for one experiment at a MOI of 2. .. Cryopreserved CD4/CD8 selected lymphocytes either from healthy donor or patient were thawed on day 0, and cultured at 2 × 10 6 /mL and activated for 48 h in AIM-V media (Gibco, Grand Island, NY) containing 5% heat inactivated human AB serum (Valley Biomedical, Winchester, VA), 2 mM Glutamax (Gibco), 40 IU/mL IL2 (Prometheus Laboratory, Inc. San Diego, CA) and stimulated with CD3/CD28 Dynabeads (Invitrogen, Camarillo, Ca) with a ratio of 1 cell: 3 beads. .. On day 2, cells were transduced with CD19/22 Bispecific CAR, FGFR4 CAR or CD22 CAR lentiviral vectors (Lentigen Technology, Gaithersburg, MD) either using bag spinoculation or Sepax spinoculation.

Article Title: High efficiency closed-system gene transfer using automated spinoculation.
Article Snippet: The CD22 CAR (Lentigen Technology, Gaithersburg, MD) was used for one experiment at a MOI of 2. .. Cell culture and lentiviral vector transduction Cryopreserved CD4/CD8 selected lymphocytes either from healthy donor or patient were thawed on day 0, and cultured at 2 × 106/mL and activated for 48 h in AIM-V media (Gibco, Grand Island, NY) containing 5% heat inactivated human AB serum (Valley Biomedical, Winchester, VA), 2 mM Glutamax (Gibco), 40 IU/mL IL2 (Prometheus Laboratory, Inc. San Diego, CA) and stimulated with CD3/CD28 Dynabeads (Invitrogen, Camarillo, Ca) with a ratio of 1 cell: 3 beads. .. On day 2, cells were transduced with CD19/22 Bispecific CAR, FGFR4 CAR or CD22 CAR lentiviral vectors (Lentigen Technology, Gaithersburg, MD) either using bag spinoculation or Sepax spinoculation.

Derivative Assay:

Article Title: NK cell compositions and preparations for immunotherapy and methods for their production
Article Snippet: .. To avoid the use of animal derived serum, an alternate protocol for the Phase 2 culture (differentiation) to produce an NK cell preparation involved the replacement of fetal bovine serum (FBS) with heat inactivated human AB serum (Valley Biomedical) or with human platelet lysate (Mill Creek Life Sciences PLTMax). ..

Irradiation:

Article Title: iPSC-derived NK cells expressing high-affinity IgG Fc receptor fusion CD64/16A to mediate flexible, multi-tumor antigen targeting for lymphoma
Article Snippet: NK cells were enriched using a negative selection human NK Cell Isolation Kit (Miltenyi Biotec, Bergisch, Germany). .. PBNK cells were expanded for 2 weeks using irradiated K562-mbIL21–41BBL feeder cells ( , ) at a 1:2 NK cell-to-feeder cell ratio in B0 media containing; DMEM and Ham’s F-12 (2:1 ratio) (Corning), 10% heat inactivated human AB serum (Valley Biomedical, Winchester), 1% penicillin-streptomycin (Gibco), 20 μM β-mercaptoethanol (MilliporeSigma), 10 μg/ml ascorbic acid (MilliporeSigma), 1.5 ng/ml sodium selenite (MilliporeSigma), 50 μM ethanolamine (MilliporeSigma), and 10 mM HEPES (Gibco). .. PBNK cells were expanded for 2 weeks using irradiated K562-mbIL21–41BBL feeder cells ( , ) at a 1:2 NK cell-to-feeder cell ratio in B0 media containing; DMEM and Ham’s F-12 (2:1 ratio) (Corning), 10% heat inactivated human AB serum (Valley Biomedical, Winchester), 1% penicillin-streptomycin (Gibco), 20 μM β-mercaptoethanol (MilliporeSigma), 10 μg/ml ascorbic acid (MilliporeSigma), 1.5 ng/ml sodium selenite (MilliporeSigma), 50 μM ethanolamine (MilliporeSigma), and 10 mM HEPES (Gibco).

Plasmid Preparation:

Article Title: High efficiency closed-system gene transfer using automated spinoculation.
Article Snippet: The CD22 CAR (Lentigen Technology, Gaithersburg, MD) was used for one experiment at a MOI of 2. .. Cell culture and lentiviral vector transduction Cryopreserved CD4/CD8 selected lymphocytes either from healthy donor or patient were thawed on day 0, and cultured at 2 × 106/mL and activated for 48 h in AIM-V media (Gibco, Grand Island, NY) containing 5% heat inactivated human AB serum (Valley Biomedical, Winchester, VA), 2 mM Glutamax (Gibco), 40 IU/mL IL2 (Prometheus Laboratory, Inc. San Diego, CA) and stimulated with CD3/CD28 Dynabeads (Invitrogen, Camarillo, Ca) with a ratio of 1 cell: 3 beads. .. On day 2, cells were transduced with CD19/22 Bispecific CAR, FGFR4 CAR or CD22 CAR lentiviral vectors (Lentigen Technology, Gaithersburg, MD) either using bag spinoculation or Sepax spinoculation.

Transduction:

Article Title: High efficiency closed-system gene transfer using automated spinoculation.
Article Snippet: The CD22 CAR (Lentigen Technology, Gaithersburg, MD) was used for one experiment at a MOI of 2. .. Cell culture and lentiviral vector transduction Cryopreserved CD4/CD8 selected lymphocytes either from healthy donor or patient were thawed on day 0, and cultured at 2 × 106/mL and activated for 48 h in AIM-V media (Gibco, Grand Island, NY) containing 5% heat inactivated human AB serum (Valley Biomedical, Winchester, VA), 2 mM Glutamax (Gibco), 40 IU/mL IL2 (Prometheus Laboratory, Inc. San Diego, CA) and stimulated with CD3/CD28 Dynabeads (Invitrogen, Camarillo, Ca) with a ratio of 1 cell: 3 beads. .. On day 2, cells were transduced with CD19/22 Bispecific CAR, FGFR4 CAR or CD22 CAR lentiviral vectors (Lentigen Technology, Gaithersburg, MD) either using bag spinoculation or Sepax spinoculation.



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